By Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)
Confocal microscopy with laser scanning know-how yields in-vivo pictures of ocular and ocular adnexal surfaces which are so great that they rival histology when it comes to quality.This distinctive atlas and textbook demonstrates general in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies a number of mobile constructions utilizing cell-density calculations and establishes correlations among novel optical sections of varied ailments of the ocular floor and scientific findings. moreover, it helps the translation of novel high-magnification optical sections via evaluating corneal and conjunctival imprint cytology with in-vivo photos and describes early inflammatory adjustments in corneal grafts, in addition to corneal conjunctivalisation in limbal stem mobile deficiency, corneal dystrophies or infections, flap interface and margin features after laser in-situ keratomileusis (LASIK). furthermore, it instructs the reader approximately diagnostic and healing follow-up ideas and gives a quick creation to functions in different fields similar to dentistry and ear, nostril and throat surgery.
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Additional resources for Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology
It is 6–10 mm thick. On confocal microscopy it is defined optically by the easily identifiable endothelial cells. The endothelium consists of about 500,000 hexagonal cells approximately 20 mm in diameter and 5 mm thick and with large, flattened central nuclei. The high concentration of cell organelles is indicative of very intensive metabolic activity. 4 Ex Vivo Applications Ex vivo confocal imaging using complex microscope systems has become a prominent and integral feature of modern-day scientific technology.
B After treatment with top- pression cytology and immunostaining of a filament. 2 Epithelium a a b b c c Fig. 24 Allergic keratoconjunctivitis: vernal keratoconjunctivitis. a Slit-lamp photograph of a 16-yearold boy with severe vernal keratoconjunctivitis causing corneal desiccation and corneal cell desquamation. b Confocal in vivo microscopy showing corneal epithelial metaplasia and hyperreflective desquamating superficial corneal cells. c Impression cytology image confirming corneal metaplasia Fig.
C Confocal in vivo microscopy image of the scar after surgical scraping of the plaque 45 46 Chapter 5 Confocal Laser Scanning In Vivo Microscopy a b c d Fig. 26 Vernal keratoconjunctivitis. a Slit-lamp photograph of a 13-year-old boy with vernal keratoconjunctivitis: Trantas dots. b Confocal in vivo microscopy image of the limbus. Epithelial infiltration by dendritic cells. c Confocal in vivo microscopy im- age of Trantas dots: microcysts and inflammatory cells (hyperreflective cells). 2 Epithelium a a b b Fig.
Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology by Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)