By Dr. Heidi Felix, Dr. Gisela Haemmerli, Prof. Dr. Peter Sträuli (auth.)
Dynamic Morphology is the try to correlate floor architec ture and form of mounted cells, as visualized by way of scanning electron microscopy (SEM), with the habit of dwelling cells, recorded by means of microcinematography (MCM). If SEM and MCM are used at the same time for the research of phone populations, a dynamic inter pretation of SEM pictures is just legitimate if the experimental stipulations are exact for the 2 innovations. this can be accomplished by means of permitting the cells to decide on a pitcher floor the place they continue to be lengthy adequate to accomplish their numerous actions less than stipulations exact for either recommendations (for technical info see Methodology). The research of a inhabitants necessitates the learn of a big variety of cells. This prerequisite is met by means of working the scanning electron micro scope at low degrees of magnification, and through the use of tradition chambers for cinematography. it may be argued that the examina tion of connected cells excludes an entire SEM survey of a inhabitants, as cells no longer adhering from the outset or turning into indifferent in the course of the diverse preparatory steps are misplaced. For this, cinematography proved to be a competent keep an eye on: All cellphone kinds well-known in time-lapse motion pictures have been additionally visible in scanning electron (SE) micrographs. one other, and extra normal, objection to a dynamic interpretation issues the artificiality of mobile habit on glass. this is often real, yet doesn't invalidate compara tive reviews utilizing this substrate.
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Extra info for Dynamic Morphology of Leukemia Cells: A Comparative Study by Scanning Electron Microscopy and Microcinematography
Slow locomotion occurs, but very infrequently. Reexamination of the peripheral blood 40 h after therapy had started has given no indication for effects on either shape or behavior of the leukemia cells. Although cells in the spheric configuration cannot be identified by SEM, they are termed myeloblasts because of their large proportion in the peripheral blood of this patient. Figure 14. [ the spheric cells. [ the sUI/ace extensions vary from one cell to the other SEM: x 9500 Figure 15. Leukemic myeloblasts (before onset of therapy) (see page 30) The large veils shown in the SEM photographs (a and c) indicate sUI/ace motility.
The arrow indicates the direction of movement a SEM: x 15,600 b TEM: x 10,200 Figure 26. 2 ~mlmin. f ca 30 ~m in 13 and 10 min respectively a SEM: x 16,400 44 b Drawing from a time-lapse film sequence a b 46 a LEUKEMIC MYELOBLASTS AML: A . L. 3 fJm /min 13 . 3 min 3 . 0 min 47 Figure 27. Leukemic myeloblasts A special feature of the polarized myeloblasts of this population is shown in the two SEM photographs (a, b): the tendency to make contact with the front while the posterior part is lifted into the medium.
A similar picture is presented by a third cell that is slightly polarized by the extension of a short foot fastened to the substrate by means of attachment filaments b Folds and veils are suggestive of sUI/ace motility a SEM: x 7300 b SEM: x 12,700 36 37 AML Case 3 This population consists of small-sized spheric cells with surface motility as the main activity. Few cells only show on-spot motility and locomotion. Figure 20. Leukemic population The sUI/ace architecture of the d(fJerent cells varies: there are microvilli, ridges, and folds.
Dynamic Morphology of Leukemia Cells: A Comparative Study by Scanning Electron Microscopy and Microcinematography by Dr. Heidi Felix, Dr. Gisela Haemmerli, Prof. Dr. Peter Sträuli (auth.)