By J. N. Hawthorne, F. A. Millar (auth.), Lloyd A. Horrocks, Louis Freysz, Gino Toffano (eds.)
The function of this ebook is to explain the newest findings when it comes to biochemical and molecular pharmacology of the fearful approach and phospholipids and to file the lawsuits of the fourth symposium on phospholipids. those Symposia were satellite tv for pc conferences of the overseas Society for Neurochemistry. This assembly used to be hung on may perhaps 26-29, 1985 within the Teatro Bibiena in Mantova, Italy. previous conferences have been held in Cortona, Italy in 1975, in Birmingham, England in 1981, and in British Columbia, Canada in 1983. As used to be the case for the court cases of these conferences, this quantity offers details that's new and critical from the researchers so much in volved in advancing our wisdom of the functionality of membranes and of lipid metabo lism within the anxious procedure. The presence of phosphorus within the mind was once said in 1719 by way of Hensing on the college of Giessen in Germany. Tower! has translated this pioneering paintings. The really lengthy, philosophical preface includes the subsequent paragraph. despite what can be considered this subject, the mind is definitely that a part of the animate physique during which that subtlest and such a lot penetrating [substance] by myself is bought, so usually circulated from the guts and cleansed through the remainder viscera, and firmly held the place thereaf ter lifestyles prospers and the motions of the reduce components endure.
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Additional info for Phospholipid Research and the Nervous System: Biochemical and Molecular Pharmacology
1977). , 1985). NGF-induced differentiation implies also extensive physiological modifications of PC12 cells. , 1982); receptors for some peptides and neurotoxins are also increased in density. Of particular interest for our work is the behaviour of cholinergic receptors. Many, but not all, PC12 subclones possess nicotinic receptors (Greene and Tischler, 1982). Muscarinic receptors increase considerably in number (about 15 fold when calculated per single cell) after NGF-induced differentiation (Jumblatt and Tischler, 1982).
In order to solve this problem we employed a direct approach. PC12 were suspended in a Ca2 +-free, EGTA-containing medium and then exposed to ionomycin. Because of the very low [Ca 2 +]o' and because of the Ca2 + ionophore-induced discharge of Ca2 + stored within cytoplasmic organelles, neither influx nor redistribution are possible in those experimental conditions. Thus, application of carbachol failed to raise [Ca2 +]j (as shown by quin-2 measurements), yet phosphoinositide hydrolysis, in particular InsP3 generation, was still stimulated.
Joseph SK and Williams RJ (1985) FEBS Lett 180: 150-154. Kirk CJ, Rodrigues LM and Hems DA (1979) Biochem J 178: 493-496. 18 Kirk CJ, Michell RH and Hems DA (1981) Biochem J 194: 155-165. Kirk CJ, Guillon G, Balestre M-N, Creba JA, Michell RH and Jard S (1985) Biochimic 67: 1161-1167. Lapetina EG, Brown WE and Michell RH (1976) J Neurochem 26: 649-651. Larrabee MG (1968) J Neurochem 26: 649-651. Larrabee MG and Leicht WS (1965) J Neurochem 12: 1-13. Larrabee MG, Klingman JD and Leicht WS (1963) J Neurochem 10: 549-560.
Phospholipid Research and the Nervous System: Biochemical and Molecular Pharmacology by J. N. Hawthorne, F. A. Millar (auth.), Lloyd A. Horrocks, Louis Freysz, Gino Toffano (eds.)